DHEA诱导雌性大鼠PCOS模型早期性器官及其相关激素变化的研究

doi:10.11843/j.issn.0366-6964.2015.08.010

Abstract

Abstract:

This experiment was designed to study the effects of dehydroeplandrosterone (DHEA) on reproductive organs development，hormones level in serum and AMH mRNA expression with the development of rat polycystic ovarian syndrome (PCOS).Eighty immature female SD rats(21 d) were randomly divided into 2 groups：control group with no DHEA(n=40) and treatment group with DHEA(6 mg•（100 g body weight）^-1，n=40).Rats were injected daily with 0.2 mL seame oil for up to 20 days.Ten rats of each groups were killed at 5，10，15 and 20 days.The blood samples were obtained，and organ indexes were calculated by weights of body，ovary，uterus，livers，kidney，and spleen.The detection results showed that the weights of body (15，20 days)，uterus (5，10，15，20 days) and ovarian (5，10 days)of treatment groups were significantly higher than the control groups (P<0.01)；Serum AMH and T of treatment groups (5，10，15，20 days) were significantly higher than control groups(P<0.01 or P<0.05)，while the serum P (15，20 days)，LH and E₂ (20 days) were higher (P<0.01 or P<0.05)；The levels of AMH mRNA of treatment groups(5，10 days) were obvious different significantly higher (P<0.05).These results indicated that the sexual organs were given priority to development during early stage of prepuberty in treated group.The abnormal AMH production could be cause of pathogenesis and development marker of PCOS in rats.The expression of AMH mRNA in ovary and serum AMH level were not exactly the same，the results may supply the reference data for further research on PCOS in female rats.

CLC Number:

S852.23

GUO Bao-ping，XU Ming-long，HU Jin，XU Yin-xue. Studies on Changes of Sexual Organs and Their Related Hormones during the Early Phase in Dehydroepiandrosterone-induced Female Rat Polycystic Ovarian Syndrome Model[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(8): 1348-1355.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2015.08.010

https://www.xmsyxb.com/EN/Y2015/V46/I8/1348

References

［1］RUTKOWSKA A，RACHON D.Bisphenol A (BPA) and its potential role in the pathogenesis of the polycystic ovary syndrome (PCOS)［J］.Gynecol Endocrinol，2014，30(4)：260-265.
［2］ERIKSEN M B，NIELSEN M F，BRUSGAARD K，et al.Genetic alterations within the DENND1A gene in patients with polycystic ovary syndrome (PCOS)［J］.PLoS ONE，2013，8(9)：e77186.
［3］DIAMANTI-KANDARAKIS E，CHRISTAKOU C，MARINAKIS E.Phenotypes and enviromental factors：their influence in PCOS［J］.Curr Pharm Des，2012，18(3)：270-282.
［4］KEDEM A，HOURVITZ A，YUNG Y，et al.Anti-Mullerian hormone (AMH) downregulation in late antral stages is impaired in PCOS patients.A study in normo-ovulatory and PCOS patients undergoing in vitro maturation (IVM) treatments［J］.Gynecol Endocrinol，2013，29(7)：651-656.
［5］KOTANIDIS L，ASIMAKOPOULOS B，NIKOLETTOS N.Association between AMH，oocyte number and availability of embryos for cryopreservation in IVF［J］.In vivo，2013，27(6)：877-880.
［6］PEIGNE M，DECANTER C.Serum AMH level as a marker of acute and long-term effects of chemotherapy on the ovarian follicular content：a systematic review［J］.Reprod Biol Endocrinol，2014，12：26.
［7］LEE M T，ANDERSON E，LEE G Y.Changes in ovarian morphology and serum hormones in the rat after treatment with dehydroepiandrosterone［J］.Anat Rec，1991，231(2)：185-192.
［8］KONG J S，YOO S A，KIM J W，et al.Anti-neuropilin-1 peptide inhibition of synoviocyte survival，angiogenesis，and experimental arthritis［J］.Arthritis Rheum，2010，62(1)：179-190.
［9］BRAWER J R，MUNOZ M，FAROOKHI R.Development of the polycystic ovarian condition (PCO) in the estradiol valerate-treated rat［J］. Biol Reprod，1986，35(3)：647-655.
［10］HOMBURG R，CRAWFORD G.The role of AMH in anovulation associated with PCOS：a hypothesis［J］.Hum Reprod，2014，29(6)：1117-1121.
［11］周宁，张海华，吕智超，等.饲粮锌水平对冬毛期水貂血清生化指标和脏器指数的影响［J］.畜牧兽医学报，2014，45（12）：1988-1994.
ZHOU N，ZHANG H H，LÜ Z C，et al.Effects of diets with different zinc levels on the selected serum biochemical indexes and organ indexes of minks during the winter hair period［J］.Acta Veterinaria et Zootechnica Sinica，2014，45(12)：1988-1994.（in Chinese）
［12］ANDERSON A D，SOLORZANO C M，MCCARTNEY C R.Childhood obesity and its impact on the development of adolescent PCOS［J］.Semin Reprod Med，2014，32(3)：202-213.
［13］王波，姜成钢，纪守坤，等.日粮尿素水平对肉羊血液学、血清学指标和消化器官的影响［J］.畜牧兽医学报，2014，45（9）：1449-1456.
WANG B，JIANG C G，JI S K，et al.Effect of different levels of urea in diets on haematological indices，serological parameters and tissue lesions in Mutton sheep［J］.Acta Veterinaria et Zootechnica Sinica，2014，45(9)：1449-1456.（in Chinese）
［14］韩芸，参芪胶囊改善PCOS子宫内膜容受性的临床与试验研究［D］.广州：广州中医药大学，2013.
HAN Y.The clinical and experimental study on improvement endometrial receptivity effects of Shen-Qi capsule in polycystic ovary syndrome［D］.Guangzhou：Guangzhou University of Chinese Medicine，2013.（in Chinese）
［15］SHAFIEE M N，KHAN G，ARIFFIN R，et al.Preventing endometrial cancer risk in polycystic ovarian syndrome (PCOS) women：could metformin help?［J］.Gynecol Oncol，2014，132(1)：248-253.
［16］GIUDICE L C.Endometrium in PCOS：Implantation and predisposition to endocrine CA［J］.Best Pract Res Clin Endocrinol Metab，2006，20(2)：235-244.
［17］张宝乐.猪GPR3基因特征、表达及其对卵泡颗粒细胞的影响［D］.南京：南京农业大学，2012.
ZHANG B L.Characterization，expression of porcine GPR3 gene and its effects on porcine follicular granulosa cells［D］.Nanjing： Nanjing Agricultural University，2012.（in Chinese）
［18］汤娟.多囊卵巢综合征（PCOS）的诊断及中医对其病理生理认识的研究［D］.长沙：湖南中医药大学，2014.
TANG J.Study on the clinical diagnosis of PCOS and the understanding of the pathophysiology of its in TCM［D］.Changsha：Hunan University of Chinese Medicine，2014.（in Chinese）
［19］TAYLOR A E，MCCOURT B，MARTIN K A，et al.Determinants of abnormal gonadotropin secretion in clinically defined women with polycystic ovary syndrome［J］.J Clin Endocrinol Metab，1997，82(7)：2248-2256.
［20］BATTAGLIA C，NAPPI R E，MANCINI F，et al.PCOS and urethrovaginal space：3-D volumetric and vascular analysis［J］.J Sex Med，2010，7(8)：2755-2764.
［21］沈宗奇.多囊卵巢综合征卵泡发育异常的相关因素及机制研究［D］.上海：复旦大学，2009.
SHEN Z Q.Correlation factors and mechanism involved in the developmental anomaly of follieles in polyeystic ovary syndrome［D］.Shanghai：Fudan University，2009.（in Chinese）
［22］郭芳，罗国静，鞠志花，等.SPEF2基因可变剪切体和功能性SNP鉴定及其与公牛精液性状的相关性研究［J］.南京农业大学学报，2014，37(3)：119-125.
GUO F，LUO G J，JU Z H，et al.Association of SPEF2 gene splices variant and functional SNP with semen quality traits in Chinese Holstein bulls［J］.Journal of Nanjing Agricultural University，2014，37(3)：119-125.（in Chinese）
［23］IMHOFF F M，YANG D，MATHEW S F，et al.The type 2 anti-Müllerian hormone receptor has splice variants that are dominant-negative inhibitors［J］.FEBS Lett，2013，587(12)：1749-1753.
［24］徐燕颖.抗苗勒氏管激素在多囊卵巢综合征中的表达及相关机制研究［D］.天津：天津医科大学，2013.
XU Y Y，The expression and related mechanism of anti-Müllerian hormone in polycystic ovary syndrome［D］.Tianjin：Tianjin Medical University，2013.（in Chinese）
［25］XU P，SHEN S M，ZHANG X L，et al.Haplotype analysis of single nucleotide polymorphisms in anti-Müllerian hormone gene in Chinese PCOS women［J］.Arch Gynecol Obstet，2013，288(1)：125-130.
［26］ESSAH P A，NESTLER J E，CARMINA E.Differences in dyslipidemia between American and Italian women with polycystic ovary syndrome［J］.J Endocrinol Invest，2008，31(1)：35-41.
［27］YU N E，HO P C.Polycystic ovary syndrome in Asian women［J］.Semin Reprod Med，2008，26(1)：14-21.
［28］SHI Y，GUO M，YAN J，et al.Analysis of clinical characteristics in large-scale Chinese women with polycystic ovary syndrome［J］.Neuro Endocrinol Lett，2007，28(6)：807-810.

[1]	JIN Meilin, LI Taotao, SUN Dongxiao, WEI Caihong. Research Progress of Epigenetic Regulation in Fat Deposition Mechanism of Livestock and Poultry [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 855-867.
[2]	LIU Ying, WANG Huanhuan, YAN Shuping, ZHU Bin, ZHANG Yuanshu. Study on the Interaction of Two Pathways of Renin Angiotensin System in Rat Nonalcoholic Simple Fatty Liver [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(11): 2309-2317.
[3]	CHEN Miaomiao, TONG Xishuai, ZHENG Jiaming, ZHAO Hongyan, GU Jianhong, LIU Zongping. Effects of 5-amino-imidazole-4-carboxamide Nucleotide on Autophagy and Differentiation in Osteoclasts [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(11): 2339-2347.
[4]	LI Zhiqiang, YAN Shuping, JI Xiaoxia, WANG Kai, ZHANG Yuanshu. Distribution and Expression of Angiotensin-Converting Enzyme 2 (ACE2) in the Jejunum of Piglets and Its Relationship with Substance Transport [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(8): 1676-1684.
[5]	WEI Yu-shuang, LIU Jiao-yang, HAN Ying-qian, GUO Zhen-zhen, LIU Xiao-he, BA Gen, HAN Li-qiang, WANG Jiang, CHU Bei-bei, YANG Guo-yu. Construction of shRNA Library of Chlorocebus Sabaeus Cathepsin Genes and the Establishment of Its Stable Transfection Cell Lines [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(6): 1282-1290.
[6]	LI Long-long, PENG Meng-ling, WANG Dian, YU Lei, GE Chong-yang, MA Hai-tian. The Effect of (一)-Hydroxycitric Acid on Proliferation and Mitochondria Function in Cultured Primary Chicken Hepatocytes [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(5): 945-953.
[7]	NIE Pei-ting，TANG Cheng，YUE Hua. Activation of Cold-inducible RNA-binding Protein by H1N1 Influenza Virus Contributes to Viral Replication Via Activating NF-κB Pathway [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(10): 2108-2114.
[8]	NIE Pei-ting，TANG Cheng，YUE Hua. Cold-inducible RNA Binding Protein of Mice Involved in Response to Influenza A Virus Infection in Mice [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(8): 1652-1657.
[9]	QUAN Su-yu，ZHANG Yuan-shu，BU Deng-pan . Heat Stress-induced Cell Injury and Effects on the Gene Expression of Milk Synthesis-related Transporters in Dairy Cow [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(8): 1704-1713.
[10]	CHAI Ji-tian，JIANG Jun-na，LI Dong，WANG Jian-bing，ZHOU Jia-hua，LI Ying-ying，WANG Zhi-qiang. Analysis of CRE and SRE Response Element in the Porcine CCR1 and CCR5 Signal Pathway [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(9): 1650-1655.
[11]	PAN Hong-bin，ZHAO Su-mei，SUN Ze-wei，LONG Guo-hui，GAO Shi-zheng，QIN Gui-xin. Fat-specific Protein 27 (Fsp27) and Lipid Metabolism [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(3): 347-353.
[12]	YANG Fan, TANG Cheng, YUE Hua. Interference of Chicken Heat Shock Protein 70 Gene Expression Induced ChickenEmbryo Fibroblast Apoptosis [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(3): 459-464.
[13]	HAN Haozhe, TIE Zihang, PANG Weijun, CAI Rui. Advances of IGF2BP2-Mediated m⁶A Modification on Animal Fat Deposition [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3605-3612.

Studies on Changes of Sexual Organs and Their Related Hormones during the Early Phase in Dehydroepiandrosterone-induced Female Rat Polycystic Ovarian Syndrome Model

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 13

Recommended Articles

Metrics

Comments